A high resolution, high speed mass-spectrometry (MS) instrument,

In this document, we, Karolinska Institutet, Department of Oncology-Pathology, Nordlund lab, invite vendors to submit offers for one state-of-the-art High Throughput High Resolution Mass Spectrometer System for isobaric tag Labelled Shotgun Proteomics applications, which remains the most widely used mode of analysis for discovery proteomics. We conduct IMPRINTS-CETSA analysis to elucidate cellular mechanism under cancer drug treatment, including studies of protein interaction changes and peptide regional cleavage, which require a parallel comparison of multiple biological replicates and a range of different treatments in an isobaric labeling format to give optimal data quality. To feasibly tackle the parallel and high-multiplexing proteomics analysis of many different samples, a high frequency of mass spectrum acquisition is crucial. The other critical capability of the required Mass Spectrometer is high resolution at the 120 to 140 m/z mass range so as to viably execute maximum isobaric labeling such as TMT32plex isobaric tag labelled analysis, giving superior quantitative interpretation, eventually aiming to reveal the key biological protein pathways behind cancer drug mechanism of action. The procurement includes delivery, installation of System/instruments with associated computer control and analysis software, extended equipment warranty, as well as training of personnel. Due to budget limitation the procurement includes the option of a factory refurbished instrument.