Direct purchase of the fast and sensitive quadrupole time-of-flight mass spectrometer with a Zeno trap.

Åbo Akademi University intend to purchase a mass spectrometry instrument for that will be shared by the metabolomics and proteomics units. The primary use will be for ESI applications utilizing the Zeno trap to boost MS2 sensitivity as well as providing the unique EAD fragmentation technique to derive information on the protein post-translational modifications and double bond positions in lipids structures of the analytes. The 7600 ZenoTOF provided by Sciex, is the only instrument in the market that meets all the specific and necessary technical requirements to fulfil the uncompromising needs of the acquiring department as follows: • The instrument must have tunable EAD fragmentation in order to separate compounds with isobaric features and lipid double bond positions via fragmentation, and to facilitate the localization of protein post-translational modifications on proteins (e.g. phosphorylation, glycosylation) • The instrument needs to be capable of fast screening with MS/MS scan rates exceeding 120Hz in large scale proteomics experiments • The instrument must have better identification of certain metabolites via the EI spectra generated by EAD • The instrument needs to have lossless MS2 acquisition modes through a trap function. • The instrument needs to have an ion source that can tolerate both nano and UHPLC flow rates without changing the ion source. • The instrument needs to have competitive sensitivity for single-cell proteomics and lipidomics analyses (>3000 protein identifications from ≤500pg of protein on ≤30-minute chromatographic gradients)