Full spectrum fluorescence Cell sorter and Cell Analyzer

This procurement involves two instruments: 1) one full 5-laser spectral flow cytometer cell sorter with sort-capable image analysis and 2) one five laser fluorescence-activated, spectral-enabled cell analyzer that allows researchers to choose between spectral or compensation-based cell analysis to meet different flow cytometry needs. The selected products together will offer a unique set of features that enables efficient and comprehensive high throughput proteomic characterization of cell suspensions at single-cell resolution. The proposed new instrumention will replace an outdated flow cytometry sorter that has been in use for over 13 years and an old conventional flow cytometry analyzer that has been in use for over 11 years. Both old instruments are nearing the end of their lifespan. The updated sorter instrumentation will meet current and future user needs for cell sorting by offering full spectrum fluorescence options and real-time imaging during sorting. In the biomedical research at the University of Oulu, single-cell analyses have seen a significant increase, as is generally observed in cell studies. Flow cytometry with sorting capabilities is necessary for sorting individual cells. The latest equipment available on the market can image each cell during sorting, thereby ensuring the required purity of cells for single-cell analysis and verifying the accuracy of results. Additionally, by imaging, sensitive cells can be sorted without lengthy cell staining processes, thus maintaining high yields of viable cells, ultimately improving, and expediting result availability. By this imaging system, which most likely soon will become the golden standard for flow cytometry, we ensure that research achieved in University of Oulu fulfills the current and future quality requirements. The imaging flow cytometer also expands the repertoire of imaging equipment at the core facility of Biocenter Oulu at the University of Oulu. The updated flow cytometry analyzer instrumentation will meet current and future user needs for cell analyses by offering full spectrum fluorescence as well as the conventional flow cytometry with compensation. The system will significantly improve the flexibility to perform both conventional and spectral flow cytometry analyses on a single instrument in a core facility environment. The acquisition is related to the project supported by the EAKR of the Regional Council of Northern Ostrobothnia ”Ketterät teknologiat innovatiivisten biosovellusten kehittämiseen”. Project code A80838. Funding comes 70% from the aforementioned project and 30% from the Oulu university strategic funds.